Conjugation of carbohydrates to proteins using di(triethylene glycol monomethyl ether) squaric acid ester revisited.

ABSTRACT: Properties of di(triethylene glycol monomethyl ether) squarate relevant to conjugation of carbohydrates to proteins have been reinvestigated and compared with those of dimethyl squarate. It is concluded that the commercially available, crystalline dimethyl squarate remains the most convenient and efficient reagent for conjugation of amine-containing carbohydrates to proteins by a two-step or one-pot conjugation protocol.

SUBMITTER: Xu P 

PROVIDER: S-EPMC5808912 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature