A Tool for the Import of Natural and Unnatural Nucleoside Triphosphates into Bacteria.

ABSTRACT: Nucleoside triphosphates play a central role in biology, but efforts to study these roles have proven difficult because the levels of triphosphates are tightly regulated in a cell and because individual triphosphates can be difficult to label or modify. In addition, many synthetic biology efforts are focused on the development of unnatural nucleoside triphosphates that perform specific functions in the cellular environment. In general, both of these efforts would be facilitated by a general means to directly introduce desired triphosphates into cells. Previously, we demonstrated that recombinant expression of a nucleoside triphosphate transporter from Phaeodactylum tricornutum (PtNTT2) in Escherichia coli functions to import triphosphates that are added to the media. Here, to explore the g

SUBMITTER: Feldman AW 

PROVIDER: S-EPMC5809120 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature