Phenotypic diversity and temporal variability in a bacterial signaling network revealed by single-cell FRET.

ABSTRACT: We present in vivo single-cell FRET measurements in the Escherichia coli chemotaxis system that reveal pervasive signaling variability, both across cells in isogenic populations and within individual cells over time. We quantify cell-to-cell variability of adaptation, ligand response, as well as steady-state output level, and analyze the role of network design in shaping this diversity from gene expression noise. In the absence of changes in gene expression, we find that single cells demonstrate strong temporal fluctuations. We provide evidence that such signaling noise can arise from at least two sources: (i) stochastic activities of adaptation enzymes, and (ii) receptor-kinase dynamics in the absence of adaptation. We demonstrate that under certain conditions, (ii) can generate giant flu

SUBMITTER: Keegstra JM 

PROVIDER: S-EPMC5809149 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature