Dicloxacillin induces CYP2C19, CYP2C9 and CYP3A4 in vivo and in vitro.

ABSTRACT:

Aim

The aim of this study was to study potential cytochrome P450 (CYP) induction by dicloxacillin.

Methods

We performed an open-label, randomized, two-phase, five-drug clinical pharmacokinetic cocktail crossover study in 12 healthy men with and without pretreatment with 1 g dicloxacillin three times daily for 10 days. Plasma and urine were collected over 24 h and the concentration of all five drugs and their primary metabolites was determined using a liquid chromatography coupled to triple quadrupole mass spectrometry method. Cryopreserved primary human hepatocytes were exposed to dicloxacillin for 48 h and changes in gene expression and the activity of CYP3A4, CYP2C9, CYP2B6 and CYP1A2 were investigated. The activation of nuclear receptors by dicloxacillin was assessed usin

SUBMITTER: Stage TB 

PROVIDER: S-EPMC5809358 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature