Quantitative Morphometry for Osteochondral Tissues Using Second Harmonic Generation Microscopy and Image Texture Information.

ABSTRACT: Osteoarthritis (OA) is a chronic joint disorder involving degeneration of articular cartilage and subchondral bone in joints. We previously established a second harmonic generation (SHG) imaging technique for evaluating degenerative changes to articular cartilage in an OA mouse model. SHG imaging, an optical label-free technique, enabled observation of collagen fibrils, and characterized critical changes in the collagenous patterns of the joints. However, it still remains to be determined how morphological changes in the organization of tissue collagen fibrils should be quantified. In this study, we addressed this issue by employing an approach based on texture analysis. Image texture analysis using the gray level co-occurrence matrix was explored to extract image features. We investigated

SUBMITTER: Saitou T 

PROVIDER: S-EPMC5809560 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature