TRPV4 regulates migration and tube formation of human retinal capillary endothelial cells.

ABSTRACT:

Background

Ca²⁺ entry plays an important role in modulating endothelial cell migration and tube formation. Transient receptor potential cation channel subfamily V member 4 (TRPV4) is a Ca²⁺-permeable channel that is widely expressed in endothelial cells. It has been reported that TRPV4 is expressed in HRCECs and regulates Ca²⁺ entry. However, the function of TRPV4 in human retinal capillary endothelial cells (HRCECs) remains unknown.

Methods

In this study we used western blot and immunostaining assay to verify TRPV4 expression in HRCECs. And then we pretreated HRCECs with HC067047 and transfected with specific shRNA of TRPV4. The functional presence of TrpV4 was determined by using fluorescence, migration and tube formation assay in TrpV4 kno

SUBMITTER: Wen L 

PROVIDER: S-EPMC5809855 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature