Basic Characterization of Natural Transformation in a Highly Transformable Haemophilus parasuis Strain SC1401.

ABSTRACT: Haemophilus parasuis causes Glässer's disease and pneumonia, incurring serious economic losses in the porcine industry. In this study, natural competence was investigated in H. parasuis. We found competence genes in H. parasuis homologous to ones in Haemophilus influenzae and a high consensus battery of Sxy-dependent cyclic AMP (cAMP) receptor protein (CRP-S) regulons using bioinformatics. High rates of natural competence were found from the onset of stationary-phase growth condition to mid-stationary phase (OD₆₀₀ from 0.29 to 1.735); this rapidly dropped off as cells reached mid-stationary phase (OD₆₀₀ from 1.735 to 1.625). As a whole, bacteria cultured in liquid media were observed to have lower competence levels than those grown on solid

SUBMITTER: Dai K 

PROVIDER: S-EPMC5809987 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature