Antisense transcription-dependent chromatin signature modulates sense transcript dynamics.

ABSTRACT: Antisense transcription is widespread in genomes. Despite large differences in gene size and architecture, we find that yeast and human genes share a unique, antisense transcription-associated chromatin signature. We asked whether this signature is related to a biological function for antisense transcription. Using quantitative RNA-FISH, we observed changes in sense transcript distributions in nuclei and cytoplasm as antisense transcript levels were altered. To determine the mechanistic differences underlying these distributions, we developed a mathematical framework describing transcription from initiation to transcript degradation. At GAL1, high levels of antisense transcription alter sense transcription dynamics, reducing rates of transcript production and processing, while incre

SUBMITTER: Brown T 

PROVIDER: S-EPMC5810148 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature