Causal effect of smoking on DNA methylation in peripheral blood: a twin and family study.

ABSTRACT:

Background

Smoking has been reported to be associated with peripheral blood DNA methylation, but the causal aspects of the association have rarely been investigated. We aimed to investigate the association and underlying causation between smoking and blood methylation.

Methods

The methylation profile of DNA from the peripheral blood, collected as dried blood spots stored on Guthrie cards, was measured for 479 Australian women including 66 monozygotic twin pairs, 66 dizygotic twin pairs, and 215 sisters of twins from 130 twin families using the Infinium HumanMethylation450K BeadChip array. Linear regression was used to estimate associations between methylation at ~ 410,000 cytosine-guanine dinucleotides (CpGs) and smoking status. A regression-based methodology for twins, Infe

SUBMITTER: Li S 

PROVIDER: S-EPMC5810186 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature