IP_3-4 kinase Arg1 regulates cell wall homeostasis and surface architecture to promote Cryptococcus neoformans infection in a mouse model.

ABSTRACT: We previously identified a series of inositol polyphosphate kinases (IPKs), Arg1, Ipk1, Kcs1 and Asp1, in the opportunistic fungal pathogen Cryptococcus neoformans. Using gene deletion analysis, we characterized Arg1, Ipk1 and Kcs1 and showed that they act sequentially to convert IP₃ to PP-IP₅ (IP₇), a key metabolite promoting stress tolerance, metabolic adaptation and fungal dissemination to the brain. We have now directly characterized the enzymatic activity of Arg1, demonstrating that it is a dual specificity (IP₃/IP₄) kinase producing IP₅. We showed previously that IP₅ is further phosphorylated by Ipk1 to produce IP₆, which is a substrate for the synthesis of PP-IP₅ by Kcs1. Phenotypic co

SUBMITTER: Li C 

PROVIDER: S-EPMC5810508 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature