Clptm1 Limits Forward Trafficking of GABA_A Receptors to Scale Inhibitory Synaptic Strength.

ABSTRACT: In contrast with numerous studies of glutamate receptor-associated proteins and their involvement in the modulation of excitatory synapses, much less is known about mechanisms controlling postsynaptic GABA_A receptor (GABA_AR) numbers. Using tandem affinity purification from tagged GABA_AR γ2 subunit transgenic mice and proteomic analysis, we isolated several GABA_AR-associated proteins, including Cleft lip and palate transmembrane protein 1 (Clptm1). Clptm1 interacted with all GABA_AR subunits tested and promoted GABA_AR trapping in the endoplasmic reticulum. Overexpression of Clptm1 reduced GABA_AR-mediated currents in a recombinant system, in cultured hippocampal neurons, and in brain, with no effect on glycine or AMP

SUBMITTER: Ge Y 

PROVIDER: S-EPMC5810584 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature