Advancing the immunoaffinity platform AFFIRM to targeted measurements of proteins in serum in the pg/ml range.

ABSTRACT: There is a great need for targeted protein assays with the capacity of sensitive measurements in complex samples such as plasma or serum, not the least for clinical purposes. Proteomics keeps generating hundreds of biomarker candidates that need to be transferred towards true clinical application through targeted verification studies and towards clinically applicable analysis formats. The immunoaffinity assay AFFIRM (AFFInity sRM) combines the sensitivity of recombinant single chain antibodies (scFv) for targeted protein enrichment with a specific mass spectrometry readout through selected reaction monitoring (SRM) in an automated workflow. Here we demonstrate a 100 times improved detection capacity of the assay down to pg/ml range through the use of oriented antibody immobilization to mag

SUBMITTER: Sall A 

PROVIDER: S-EPMC5810979 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature