Developing diazirine-based chemical probes to identify histone modification 'readers' and 'erasers'.

ABSTRACT: Post translational modifications (PTMs, e.g., phosphorylation, acetylation and methylation) of histone play important roles in regulating many fundamental cellular processes such as gene transcription, DNA replication and damage repair. While 'writer' and 'eraser' enzymes modify histones by catalyzing the addition and removal of histone PTMs, 'reader' proteins recognize these modified histones and 'translate' the PTMs by executing distinct cellular programs. Therefore, identification of the regulating enzymes and binding partners of histone PTMs is essential for understanding their regulatory mechanisms and cellular functions. Here we report the development of diazirine-based photoaffinity probes for identification of 'readers' and 'erasers' of histone PTMs. When compared with previ

SUBMITTER: Yang T 

PROVIDER: S-EPMC5811097 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature