A hydrogel biosensor for high selective and sensitive detection of amyloid-beta oligomers.

ABSTRACT:

Background

Alzheimer's disease (AD) is a neurodegenerative disorder characterized by progressive cognitive and memory impairment. It is the most common neurological disease that causes dementia. Soluble amyloid-beta oligomers (AβO) in blood or cerebrospinal fluid (CSF) are the pathogenic biomarker correlated with AD.

Methods

A simple electrochemical biosensor using graphene oxide/gold nanoparticles (GNPs) hydrogel electrode was developed in this study. Thiolated cellular prion protein (PrP^C) peptide probe was immobilized on GNPs of the hydrogel electrode to construct an AβO biosensor. Electrochemical impedance spectroscopy was utilized for AβO analysis.

Results

The specific binding between AβO and PrP^C probes on the hydrogel electrode resulted in

SUBMITTER: Sun L 

PROVIDER: S-EPMC5811178 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature