Pan-Genotype Hepatitis E Virus Replication in Stem Cell-Derived Hepatocellular Systems.

ABSTRACT:

Background & aims

The 4 genotypes of hepatitis E virus (HEV) that infect humans (genotypes 1-4) vary in geographical distribution, transmission, and pathogenesis. Little is known about the properties of HEV or its hosts that contribute to these variations. Primary isolates grow poorly in cell culture; most studies have relied on variants adapted to cancer cell lines, which likely alter virus biology. We investigated the infection and replication of primary isolates of HEV in hepatocyte-like cells (HLCs) derived from human embryonic and induced pluripotent stem cells.

Methods

Using a cell culture-adapted genotype 3 strain and primary isolates of genotypes 1 to 4, we compared viral replication kinetics, sensitivity to drugs, and ability of HEV to activate the innate immune res

SUBMITTER: Wu X 

PROVIDER: S-EPMC5811326 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature