Effect of dC → d(m⁵C) substitutions on the folding of intramolecular triplexes with mixed TAT and C⁺GC base triplets.

ABSTRACT: Oligonucleotide-directed triple helix formation has been recognized as a potential tool for targeting genes with high specificity. Cystosine methylation in the 5' position is both ubiquitous and a stable regulatory modification, which could potentially stabilize triple helix formation. In this work, we have used a combination of calorimetric and spectroscopic techniques to study the intramolecular unfolding of four triplexes and two duplexes. We used the following triplex control sequence, named Control Tri, d(AGAGAC₅TCTCTC₅TCTCT), where C₅ are loops of five cytosines. From this sequence, we studied three other sequences with dC → d(m⁵C) substitutions on the Hoogsteen strand (2MeH), Crick strand (2MeC) and both strands (4MeHC). Calorimetric studi

SUBMITTER: Carr CE 

PROVIDER: S-EPMC5811340 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature