Identification of Chloride Channels CLCN3 and CLCN5 Mediating the Excitatory Cl^- Currents Activated by Sphingosine-1-Phosphate in Sensory Neurons.

ABSTRACT: Sphingosine-1-phosphate (S1P) is a bioactive sphingolipid involved in numerous physiological and pathophysiological processes. We have previously reported a S1P-induced nocifensive response in mice by excitation of sensory neurons via activation of an excitatory chloride current. The underlying molecular mechanism for the S1P-induced chloride conductance remains elusive. In the present study, we identified two CLCN voltage-gated chloride channels, CLCN3 and CLCN5, which mediated a S1P-induced excitatory Cl^- current in sensory neurons by combining RNA-seq, adenovirus-based gene silencing and whole-cell electrophysiological voltage-clamp recordings. Downregulation of CLCN3 and CLCN5 channels by adenovirus-mediated delivery of shRNA dramatically reduced S1P-induced Cl^- c

SUBMITTER: Qi Y 

PROVIDER: S-EPMC5811518 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature