Synthetic Whole-Cell Biodevices for Targeted Degradation of Antibiotics.

ABSTRACT: Synthetic biology enables infinite possibilities in biotechnology via employing genetic modules. However, not many researches have explored the potentials of synthetic biology in environmental bioprocesses. In this study, we introduced a genetic module harboring the codon-optimized tetracycline degrading gene, tetX.co, into the model host, Escherichia coli, and generated a prototypal whole-cell biodevice for the degradation of a target antibiotic. Our results suggested that E. coli with the tetX.co-module driven by either the P_J23119 or P_BAD promoters conferred resistance up to 50 μg/mL of tetracycline and degrades over 95% of tetracycline within 24 h. The detoxification ability of tetX was further verified in conditioned media by typical E. coli K-12 and B strains as

SUBMITTER: Xia PF 

PROVIDER: S-EPMC5811551 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature