Next-generation sequencing from bulked segregant analysis identifies a dwarfism gene in watermelon.

ABSTRACT: Dwarfism is one of the most valuable traits in watermelon breeding mainly because of its contribution to yield as well as the decreased labor required to cultivate and harvest smaller plants. However, the underlying genetic mechanism is unknown. In this study, a candidate dwarfism gene was identified by applying next-generation sequencing technology to analyze watermelon plants. We completed a whole-genome re-sequencing of two DNA bulks (dwarf pool and vine pool) generated from plants in an F₂ population. A genome-wide analysis of single nucleotide polymorphisms resulted in the detection of a genomic region harboring the candidate dwarfism gene Cla010726. The encoded protein was predicted to be a gibberellin 20-oxidase-like protein, which is a well-known "green revolution" prote

SUBMITTER: Dong W 

PROVIDER: S-EPMC5811605 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature