Nanobody-Directed Specific Degradation of Proteins by the 26S-Proteasome in Plants.

ABSTRACT: Here, we present data showing the directed degradation of target proteins recognized by a specific nanobody in transgenic plants. Green fluorescent protein was depleted by a chimeric nanobody fused to a distinct F-box domain, which enables protein degradation via the ubiquitin proteasome pathway. This technique could thus be used to knock out other proteins of interest in planta using specific, high-affinity binding proteins.

SUBMITTER: Baudisch B 

PROVIDER: S-EPMC5811635 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature