Direct detection and characterization of foot-and-mouth disease virus in East Africa using a field-ready real-time PCR platform.

ABSTRACT: Effective control and monitoring of foot-and-mouth disease (FMD) relies upon rapid and accurate disease confirmation. Currently, clinical samples are usually tested in reference laboratories using standardized assays recommended by The World Organisation for Animal Health (OIE). However, the requirements for prompt and serotype-specific diagnosis during FMD outbreaks, and the need to establish robust laboratory testing capacity in FMD-endemic countries have motivated the development of simple diagnostic platforms to support local decision-making. Using a portable thermocycler, the T-COR™ 8, this study describes the laboratory and field evaluation of a commercially available, lyophilized pan-serotype-specific real-time RT-PCR (rRT-PCR) assay and a newly available FMD virus (FMDV) typing ass

SUBMITTER: Howson ELA 

PROVIDER: S-EPMC5811823 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature