Optimized isolation and expansion of human airway epithelial basal cells from endobronchial biopsy samples.

ABSTRACT: Autologous airway epithelial cells have been used in clinical tissue-engineered airway transplantation procedures with a view to assisting mucosal regeneration and restoring mucociliary escalator function. However, limited time is available for epithelial cell expansion due to the urgent nature of these interventions and slow epithelial regeneration has been observed in patients. Human airway epithelial cells can be expanded from small biopsies or brushings taken during bronchoscopy procedures, but the optimal mode of tissue acquisition from patients has not been investigated. Here, we compared endobronchial brushing and endobronchial biopsy samples in terms of their cell number and their ability to initiate basal epithelial stem cell cultures. We found that direct co-culture of samples wi

SUBMITTER: Gowers KHC 

PROVIDER: S-EPMC5811901 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature