IFN-stimulated Gene Expression, Type 2 Inflammation, and Endoplasmic Reticulum Stress in Asthma.

ABSTRACT:

Rationale

Quantification of type 2 inflammation provided a molecular basis for heterogeneity in asthma. Non-type 2 pathways that contribute to asthma pathogenesis are not well understood.

Objectives

To identify dysregulated pathways beyond type 2 inflammation.

Methods

We applied RNA sequencing to airway epithelial brushings obtained from subjects with stable mild asthma not on corticosteroids (n = 19) and healthy control subjects (n = 16). Sequencing reads were mapped to human and viral genomes. In the same cohort, and in a separate group with severe asthma (n = 301), we profiled blood gene expression with microarrays.

Measurements and main results

In airway brushings from mild asthma on inhaled corticosteroids, RNA sequencing yielded 1,379 differentially expre

SUBMITTER: Bhakta NR 

PROVIDER: S-EPMC5811952 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature