Real-time fluorescence loop-mediated isothermal amplification assay for direct detection of egg drop syndrome virus.

ABSTRACT:

Background

Egg drop syndrome (EDS), caused by the adenovirus "egg drop syndrome virus" (EDSV) causes severe economic losses through reduced egg production in breeder and layer flocks. The diagnosis of EDSV has been done by molecular tools since its complete genome sequence was identified. In order to enhance the capabilities of the real-time fluorescence loop-mediated isothermal amplification (RealAmp) assay, we aimed to apply the method for direct detection of the EDSV without viral DNA extraction. In order to detect the presence of the EDSV DNA, three pairs of primers were designed, from the conserved region of fiber gene of the EDSV.

Results

For our assay, test and control samples were directly used in the reaction mixture in 10-fold serial dilution. The target DNA was am

SUBMITTER: Zheney M 

PROVIDER: S-EPMC5811957 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature