The impact of down-regulated SK3 expressions on Hirschsprung disease.

ABSTRACT:

Background

Some Hirschsprung's disease (HSCR) patients showed persistent bowel symptoms following an appropriately performed pull-through procedure. The mechanism is presumed to be down-regulated small-conductance calcium-activated potassium channel 3 (SK3) expression in the HSCR ganglionic intestines. We aimed to investigate the SK3 expression's impact in HSCR patients after a properly performed pull-through surgery in an Indonesian population, a genetically distinct group within Asia.

Methods

We assessed SK3 gene expression in both the ganglionic and aganglionic colon of HSCR patients and controls colon by quantitative real-time polymerase chain reaction (RT-PCR).

Results

We ascertained fourteen sporadic HSCR patients and six anorectal malformation patients as contr

SUBMITTER: Gunadi 

PROVIDER: S-EPMC5811966 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature