SALP, a new single-stranded DNA library preparation method especially useful for the high-throughput characterization of chromatin openness states.

ABSTRACT:

Background

Next-generation sequencing (NGS) is fundamental to the current biological and biomedical research. Construction of sequencing library is a key step of NGS. Therefore, various library construction methods have been explored. However, the current methods are still limited by some shortcomings.

Results

This study developed a new NGS library construction method, Single strand Adaptor Library Preparation (SALP), by using a novel single strand adaptor (SSA). SSA is a double-stranded oligonucleotide with a 3' overhang of 3 random nucleotides, which can be efficiently ligated to the 3' end of single strand DNA by T4 DNA ligase. SALP can be started with any denatured DNA fragments such as those sheared by Tn5 tagmentation, enzyme digestion and sonication. When started with

SUBMITTER: Wu J 

PROVIDER: S-EPMC5811972 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature