High fidelity hypothermic preservation of primary tissues in organ transplant preservative for single cell transcriptome analysis.

ABSTRACT:

Background

High-fidelity preservation strategies for primary tissues are in great demand in the single cell RNAseq community. A reliable method would greatly expand the scope of feasible multi-site collaborations and maximize the utilization of technical expertise. When choosing a method, standardizability and fidelity are important factors to consider due to the susceptibility of single-cell RNAseq analysis to technical noise. Existing approaches such as cryopreservation and chemical fixation are less than ideal for failing to satisfy either or both of these standards.

Results

Here we propose a new strategy that leverages preservation schemes developed for organ transplantation. We evaluated the strategy by storing intact mouse kidneys in organ transplant preservative solut

SUBMITTER: Wang W 

PROVIDER: S-EPMC5811979 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature