Activatable Protein Nanoparticles for Targeted Delivery of Therapeutic Peptides.

ABSTRACT: Clinical translation of therapeutic peptides, particularly those that require penetration of the cell membrane or are cytolytic, is a major challenge. A novel approach based on a complementary mechanism, which has been widely used for guided synthesis of DNA or RNA nanoparticles, for de novo design of activatable protein nanoparticles (APNPs) for targeted delivery of therapeutic peptides is described. APNPs are formed through self-assembly of three independent polypeptides based on pairwise coiled-coil dimerization. They are capable of long circulation in the blood and can be engineered to target diseases. Peptides to be delivered are incorporated into APNPs and released into the disease microenvironment by locally enriched proteases. It is demonstrated that APNPs mediate efficient deliver

SUBMITTER: Yu X 

PROVIDER: S-EPMC5812013 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature