Simultaneous measurement of sleep and feeding in individual Drosophila.

ABSTRACT: Drosophila is widely used for the dissection of genetic and neuronal mechanisms of behavior. Recently, flies have emerged as a model for investigating the regulation of feeding and sleep. Although typically studied in isolation, increasing evidence points to a fundamental connection between these behaviors. Thus, a system for measuring sleep and feeding simultaneously in a single integrated system is important for interpreting behavioral shifts of either state. Here, we describe the construction and use of the Activity Recording Capillary Feeder or CAFE (ARC), a machine-vision (automated image tracking)-based system for the integrated measurement of sleep and feeding in individual Drosophila. Flies feed on liquid food from a microcapillary, and consumption is measured by tracking the liqui

SUBMITTER: Murphy KR 

PROVIDER: S-EPMC5812262 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature