PCR-Free, Multiplexed Expression Profiling of microRNAs Using Silicon Photonic Microring Resonators.

ABSTRACT: We describe an approach for multiplexed microRNA analysis using silicon photonic microring resonators to detect cDNA reverse transcription products via a subsequent enzymatic signal enhancement strategy. Key to this method is a modified stem loop primer that facilitates downstream signal amplification via enzymatic turnover and improves the sensor signal 20-fold when compared to traditional stem loop primers. This approach facilitates targeted microRNA quantification in only 2.5 h and without requiring target amplification via the polymerase chain reaction (PCR). Primers for 7 miRNA targets were orthogonally designed to avoid cross-hybridization between capture probes. This approach was applied to the detection of total RNA from human tissues and found to display differential expression pr

SUBMITTER: Graybill RM 

PROVIDER: S-EPMC5812356 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature