Practicable methods for histological section thickness measurement in quantitative stereological analyses.

ABSTRACT: The accuracy of quantitative stereological analysis tools such as the (physical) disector method substantially depends on the precise determination of the thickness of the analyzed histological sections. One conventional method for measurement of histological section thickness is to re-embed the section of interest vertically to its original section plane. The section thickness is then measured in a subsequently prepared histological section of this orthogonally re-embedded sample. However, the orthogonal re-embedding (ORE) technique is quite work- and time-intensive and may produce inaccurate section thickness measurement values due to unintentional slightly oblique (non-orthogonal) positioning of the re-embedded sample-section. Here, an improved ORE method is presented, allowing for dete

SUBMITTER: Matenaers C 

PROVIDER: S-EPMC5812658 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature