Development of a recombinase polymerase amplification assay for rapid detection of Francisella noatunensis subsp. orientalis.

ABSTRACT: Francisella noatunensis subsp. orientalis (Fno) is the causative agent of piscine francisellosis in warm water fish including tilapia. The disease induces chronic granulomatous inflammation with high morbidity and can result in high mortality. Early and accurate detection of Fno is crucial to set appropriate outbreak control measures in tilapia farms. Laboratory detection of Fno mainly depends on bacterial culture and molecular techniques. Recombinase polymerase amplification (RPA) is a novel isothermal technology that has been widely used for the molecular diagnosis of various infectious diseases. In this study, a recombinase polymerase amplification (RPA) assay for rapid detection of Fno was developed and validated. The RPA reaction was performed at a constant temperature of 42°C for 20

SUBMITTER: Shahin K 

PROVIDER: S-EPMC5812721 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature