Hepatitis C virus deep sequencing for sub-genotype identification in mixed infections: A real-life experience.

ABSTRACT:

Background

The effectiveness of the new generation of hepatitis C treatments named direct-acting antiviral agents (DAAs) depends on the genotype, subtype, and resistance-associated substitutions present in individual patients. The aim of this study was to evaluate a massive sequencing platform for the analysis of genotypes and subtypes of hepatitis C virus (HCV) in order to optimize therapy.

Methods

A total of 84 patients with hepatitis C were analyzed. The routine genotyping methodology for HCV used at the study institution (Versant HCV Assay, LiPA) was compared with a deep sequencing platform (454/GS-Junior and Illumina MiSeq).

Results

The mean viral load in these HCV patients was 6.89×10⁶±7.02×10⁵. Viral genotypes analyzed by LiPA were distrib

SUBMITTER: Del Campo JA 

PROVIDER: S-EPMC5812776 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature