Protein identification in imaging mass spectrometry through spatially targeted liquid micro-extractions.

ABSTRACT:

Rationale

Liquid extraction surface analysis (LESA) can be used to generate spatially directed protein identifications in an imaging mass spectrometry (IMS) workflow. This approach involves the use of robotic micro-extractions coupled to online liquid chromatography (LC). We have characterized the extraction efficiency of this method as well as its ability to identify proteins from a matrix assisted laser/desorption ionization (MALDI) IMS experiment.

Methods

Proteins and peptides were extracted from transverse sections of a rat brain and sagittal sections of a mouse pup using liquid surface extractions. Extracts were either analyzed by online LC coupled to a high mass resolution Fourier transform ion cyclotron resonance (FTICR) mass spectrometer or collected offline and anal

SUBMITTER: Ryan DJ 

PROVIDER: S-EPMC5812809 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature