Enhancing the Adaptability of the Deep-Sea Bacterium Shewanella piezotolerans WP3 to High Pressure and Low Temperature by Experimental Evolution under H₂O₂ Stress.

ABSTRACT: Oxidative stresses commonly exist in natural environments, and microbes have developed a variety of defensive systems to counteract such events. Although increasing evidence has shown that high hydrostatic pressure (HHP) and low temperature (LT) induce antioxidant defense responses in cells, there is no direct evidence to prove the connection between antioxidant defense mechanisms and the adaptation of bacteria to HHP and LT. In this study, using the wild-type (WT) strain of a deep-sea bacterium, Shewanella piezotolerans WP3, as an ancestor, we obtained a mutant, OE100, with an enhanced antioxidant defense capacity by experimental evolution under H₂O₂ stress. Notably, OE100 exhibited better tolerance not only to H₂O₂ stress but also to HHP

SUBMITTER: Xie Z 

PROVIDER: S-EPMC5812942 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature