Archimedes' principle for characterisation of recombinant whole cell biocatalysts.

ABSTRACT: The ability of whole cells to catalyse multistep reactions, often yielding synthetically demanding compounds later used by industrial biotech or pharma, makes them an indispensable tool of synthetic chemistry. The complex reaction network employed by cellular catalysts and the still only moderate predictive power of modelling approaches leaves this tool challenging to engineer. Frequently, large libraries of semi-rationally generated variants are sampled in high-throughput mode in order to then identify improved catalysts. We present a method for space- and time-efficient processing of very large libraries (10⁷) of recombinant cellular catalysts, in which the phenotypic characterisation and the isolation of positive variants for the entire library is done within one minute in a

SUBMITTER: Schmitt S 

PROVIDER: S-EPMC5813238 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature