RNA-QC-chain: comprehensive and fast quality control for RNA-Seq data.

ABSTRACT:

Background

RNA-Seq has become one of the most widely used applications based on next-generation sequencing technology. However, raw RNA-Seq data may have quality issues, which can significantly distort analytical results and lead to erroneous conclusions. Therefore, the raw data must be subjected to vigorous quality control (QC) procedures before downstream analysis. Currently, an accurate and complete QC of RNA-Seq data requires of a suite of different QC tools used consecutively, which is inefficient in terms of usability, running time, file usage, and interpretability of the results.

Results

We developed a comprehensive, fast and easy-to-use QC pipeline for RNA-Seq data, RNA-QC-Chain, which involves three steps: (1) sequencing-quality assessment and trimming; (2) internal

SUBMITTER: Zhou Q 

PROVIDER: S-EPMC5813327 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature