Extracellular ATP activates store-operated Ca²⁺ entry in white adipocytes: functional evidence for STIM1 and ORAI1.

ABSTRACT: In the present study, we have applied ratiometric measurements of intracellular Ca²⁺ concentrations ([Ca²⁺]_i) to show that extracellularly applied ATP (adenosine triphosphate) (100 µM) stimulates store-operated Ca²⁺ entry (SOCE) in 3T3-L1 adipocytes. ATP produced a rapid increase in [Ca²⁺]_i consisting of an initial transient elevation followed by a sustained elevated phase that could be observed only in the presence of extracellular Ca²⁺ Gene expression data and [Ca²⁺]_i recordings with uridine-5'-triphosphate or with the phospholipase C (PLC) inhibitor U73122 demonstrated the involvement of purinergic P2Y2 receptors and the PLC/inositol trisphosphate pathway. The [Ca²⁺]_i

SUBMITTER: El Hachmane MF 

PROVIDER: S-EPMC5813502 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature