From Otic Induction to Hair Cell Production: Pax2^EGFP Cell Line Illuminates Key Stages of Development in Mouse Inner Ear Organoid Model.

ABSTRACT: Producing hair cells of the inner ear is the major goal of ongoing research that combines advances in developmental and stem cell biology. The recent advent of an inner ear organoid protocol-resulting in three-dimensional stem cell-derived tissues resembling vestibular sensory epithelia-has sparked interest in applications such as regeneration, drug discovery, and disease modeling. In this study, we adapted this protocol for a novel mouse embryonic stem cell line with a fluorescent reporter for Pax2 expression. We used Pax2^EGFP/+ organoid formation to model otic induction, the pivotal developmental event when preplacodal tissue adopts otic fate. We found upregulation of Pax2 and activation of ERK downstream of fibroblast growth factor signaling in organoid formation as in embryo

SUBMITTER: Schaefer SA 

PROVIDER: S-EPMC5813733 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature