Vitro culture of axe-head glochidia in pink heelsplitter Potamilus alatus and mechanism of its high host specialists.

ABSTRACT: The basal media M199 or MEM was utilized in the classical method of vitro culture of glochidia where 1-5% CO2 was required to maintain stable physiological pH for completion of non-parasitic metamorphosis. The classical method encounters a great challenge to those glochidia which undergo development of visceral tissue but significantly increase in size during metamorphosis. The improved in vitro culture techniques and classical methods were firstly compared for non-parasitic metamorphosis and development of glochidia in pink heelsplitter. Based on the improved method, the optimal vitro culture media was further selected from 14 plasmas or sera, realizing the non-parasitic metamorphosis of axe-head glochidia for the first time. The results showed that addition of different plasma (serum) ha

SUBMITTER: Wen HB 

PROVIDER: S-EPMC5813935 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature