Circulating small non-coding RNAs reflect IFN status and B cell hyperactivity in patients with primary Sjogren's syndrome.

ABSTRACT:

Background

Considering the important role of miRNAs in the regulation of post-transcriptional expression of target genes, we investigated circulating small non-coding RNAs (snc)RNA levels in patients with primary Sjögren's syndrome (pSS). In addition we assessed if serum sncRNA levels can be used to differentiate patients with specific disease features.

Methods

Serum RNA was isolated from 37 pSS patients as well as 21 patients with incomplete Sjögren's Syndrome (iSS) and 17 healthy controls (HC) allocated to two independent cohorts: discovery and validation. OpenArray profiling of 758 sncRNAs was performed in the discovery cohort. Selected sncRNAs were measured in the validation cohort using single-assay RT-qPCR. In addition, unsupervised hierarchical clustering was performe

SUBMITTER: Lopes AP 

PROVIDER: S-EPMC5814054 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature