Ultrafast Peptide Label-Free Quantification with FlashLFQ.

ABSTRACT: The rapid and accurate quantification of peptides is a critical element of modern proteomics that has become increasingly challenging as proteomic data sets grow in size and complexity. We present here FlashLFQ, a computer program for high-speed label-free quantification of peptides following a search of bottom-up mass spectrometry data. FlashLFQ is approximately an order of magnitude faster than established label-free quantification methods. The increased speed makes it practical to base quantification upon all of the charge states for a given peptide rather than solely upon the charge state that was selected for MS2 fragmentation. This increases the number of quantified peptides, improves replicate-to-replicate reproducibility, and increases quantitative accuracy. We integrated FlashLFQ

SUBMITTER: Millikin RJ 

PROVIDER: S-EPMC5814109 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature