Time- and polarity-dependent proteomic changes associated with homeostatic scaling at central synapses.

ABSTRACT: In homeostatic scaling at central synapses, the depth and breadth of cellular mechanisms that detect the offset from the set-point, detect the duration of the offset and implement a cellular response are not well understood. To understand the time-dependent scaling dynamics we treated cultured rat hippocampal cells with either TTX or bicucculline for 2 hr to induce the process of up- or down-scaling, respectively. During the activity manipulation we metabolically labeled newly synthesized proteins using BONCAT. We identified 168 newly synthesized proteins that exhibited significant changes in expression. To obtain a temporal trajectory of the response, we compared the proteins synthesized within 2 hr or 24 hr of the activity manipulation. Surprisingly, there was little overlap in the signi

SUBMITTER: Schanzenbacher CT 

PROVIDER: S-EPMC5814146 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature