The involvement of serum exosomal miR-500-3p and miR-770-3p in aging: modulation by calorie restriction.

ABSTRACT: Recent studies have shown a role for miRNAs in aging and age-related diseases, and the modulation of miRNA expression by diet attracts attention as a new therapeutic strategy. Here, we focused on identifying specific exosomal miRNAs derived from serum of aged rats and the effect of short-term calorie restriction (CR) on their expression. Exosomes from serum of young (7-month), old (22-month), and old-CR Sprague Dawley rats were isolated and characterized by transmission electron microscopy analyses, dynamic light scattering measurements, and Western blotting. A total of 12 significantly expressed miRNAs in serum exosomes of young and old rats were identified by next generation sequencing. After analysis of qRT-PCR, we found that miR-500-3p and miR-770-3p expression was significantly upregu

SUBMITTER: Lee EK 

PROVIDER: S-EPMC5814159 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature