DNA bipedal motor walking dynamics: an experimental and theoretical study of the dependency on step size.

ABSTRACT: We present a detailed coarse-grained computer simulation and single molecule fluorescence study of the walking dynamics and mechanism of a DNA bipedal motor striding on a DNA origami. In particular, we study the dependency of the walking efficiency and stepping kinetics on step size. The simulations accurately capture and explain three different experimental observations. These include a description of the maximum possible step size, a decrease in the walking efficiency over short distances and a dependency of the efficiency on the walking direction with respect to the origami track. The former two observations were not expected and are non-trivial. Based on this study, we suggest three design modifications to improve future DNA walkers. Our study demonstrates the ability of the oxDNA mode

SUBMITTER: Khara DC 

PROVIDER: S-EPMC5814849 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature