Genome-wide identification of tolerance mechanisms toward p-coumaric acid in Pseudomonas putida.

ABSTRACT: The soil bacterium Pseudomonas putida KT2440 has gained increasing biotechnological interest due to its ability to tolerate different types of stress. Here, the tolerance of P. putida KT2440 toward eleven toxic chemical compounds was investigated. P. putida was found to be significantly more tolerant toward three of the eleven compounds when compared to Escherichia coli. Increased tolerance was for example found toward p-coumaric acid, an interesting precursor for polymerization with a significant industrial relevance. The tolerance mechanism was therefore investigated using the genome-wide approach, Tn-seq. Libraries containing a large number of miniTn5-Km transposon insertion mutants were grown in the presence and absence of p-coumaric acid, and the enrichment or depletion of mutants was

SUBMITTER: Calero P 

PROVIDER: S-EPMC5814926 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature