T-ARMS PCR genotyping of SNP rs445709131 using thermostable strand displacement polymerase.

ABSTRACT:

Objectives

In a recent publication, we reported the successful use of tetra primer-amplification refractory mutation system based polymerase chain reaction (T-ARMS-PCR) for genotyping of rs445709131-SNP responsible for the bovine leukocyte adhesion deficiency (BLAD) in cattle. The SNP is characterized by higher GC content of the surrounding region, hence, the previous protocol utilized dimethyl sulfoxide as PCR enhancer. Here, the reaction cocktail was modified with the use of thermostable strand displacement polymerase (SD polymerase) instead of commonly used Taq DNA Polymerase. The amplification efficiency, reaction sensitivity, specificity, and need of PCR enhancer in reactions containing SD polymerase and Taq polymerase were compared.

Results

T-ARMS-PCR assay is influenc

SUBMITTER: Alyethodi RR 

PROVIDER: S-EPMC5815177 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature