RNA secondary structure profiling in zebrafish reveals unique regulatory features.

ABSTRACT:

Background

RNA is known to play diverse roles in gene regulation. The clues for this regulatory function of RNA are embedded in its ability to fold into intricate secondary and tertiary structure.

Results

We report the transcriptome-wide RNA secondary structure in zebrafish at single nucleotide resolution using Parallel Analysis of RNA Structure (PARS). This study provides the secondary structure map of zebrafish coding and non-coding RNAs. The single nucleotide pairing probabilities of 54,083 distinct transcripts in the zebrafish genome were documented. We identified RNA secondary structural features embedded in functional units of zebrafish mRNAs. Translation start and stop sites were demarcated by weak structural signals. The coding regions were characterized by the three

SUBMITTER: Kaushik K 

PROVIDER: S-EPMC5815192 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature