Direct assessment of renal mitochondrial redox state using hyperpolarized ¹³ C-acetoacetate.

ABSTRACT:

Purpose

The purpose of this study was to investigate the hyperpolarized ketone body ¹³ C-acetoacetate (AcAc) and its conversion to ¹³ C-β-hydroxybutyrate (βOHB) in vivo, catalyzed by β-hydroxybutyrate dehydrogenase (BDH), as a novel direct marker of mitochondrial redox state.

Methods

[1,3-¹³ C₂ ]AcAc was synthesized by hydrolysis of the ethyl ester, and hyperpolarized via dissolution DNP. Cold storage under basic conditions resulted in sufficient chemical stability for use in hyperpolarized (HP) MRI studies. Polarizations and relaxation times of HP [1,3-¹³ C₂ ]AcAc were measured in a clinical 3T MRI scanner, and 8 rats were scanned by dynamic HP ¹³ C MR spectroscopy of a slab through the kidneys. Fo

SUBMITTER: von Morze C 

PROVIDER: S-EPMC5815327 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature