In vitro biocatalytic pathway design: orthogonal network for the quantitative and stereospecific amination of alcohols.

ABSTRACT: The direct and efficient conversion of alcohols into amines is a pivotal transformation in chemistry. Here, we present an artificial, oxidation-reduction, biocatalytic network that employs five enzymes (alcohol dehydrogenase, NADP-oxidase, catalase, amine dehydrogenase and formate dehydrogenase) in two concurrent and orthogonal cycles. The NADP-dependent oxidative cycle converts a diverse range of aromatic and aliphatic alcohol substrates to the carbonyl compound intermediates, whereas the NAD-dependent reductive aminating cycle generates the related amine products with >99% enantiomeric excess (R) and up to >99% conversion. The elevated conversions stem from the favorable thermodynamic equilibrium (K'_eq = 1.88 × 10⁴² and 1.48 × 10⁴¹ for the amination of pr

SUBMITTER: Knaus T 

PROVIDER: S-EPMC5815496 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature